Genetic Transformation in Rhizobium
نویسندگان
چکیده
منابع مشابه
Genetic transformation of Rhizobium leguminosarum by plasmid DNA.
We demonstrated the genetic transformation of Rhizobium leguminosarum by R68.45 plasmid DNA by freezing and thawing cell suspensions in the presence of R68.45 plasmid DNA and 20 mM MgCl2. Clones resistant to kanamycin and tetracycline were recovered at a frequency of 10(-8) per recipient cell. No colonies that were doubly drug resistant were recovered in parallel control experiments.
متن کاملHigh-frequency transformation of Rhizobium meliloti.
Transformation of R factor RP4 and its derivative pRK290 from Escherichia coli to Rhizobium meliloti is reported. The efficiency of transformation was in the range of 10(-5) per viable cell. In addition, chromosomal DNA prepared from one R. meliloti strain resistant to streptomycin was transferred to the isoleucine-valine-requiring mutant susceptible to streptomycin.
متن کاملGenetic Transformation of Rhizobium: a Review of the Work of R. Balassa.
PREFATORY NOTE........................................................ 228 INTRODUCTION.................................................................................. 229 RESEARCH ON TRANSFORMATION................................................................ 229 Strains....................................................................................... 230 Media...........................
متن کاملGenetic map of Rhizobium meliloti megaplasmid pRmeSU47b.
A circular linkage map of the Rhizobium meliloti megaplasmid pRmeSU47b was constructed. The map consists of transposon insertions carrying alternating antibiotic resistance markers linked by phi M12 transduction. Data from conjugation experiments utilizing donor strains carrying Tn5-oriT insertions in the megaplasmid supported the proposed genetic map. In addition, the positions of previously i...
متن کاملGenetic Transformation in Citrus
Citrus is one of the world's important fruit crops. Recently, citrus molecular genetics and biotechnology work have been accelerated in the world. Genetic transformation, a biotechnological tool, allows the release of improved cultivars with desirable characteristics in a shorter period of time and therefore may be useful in citrus breeding programs. Citrus transformation has now been achieved ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1969
ISSN: 0022-1287
DOI: 10.1099/00221287-57-1-125